A developmental landscape of 3D-cultured human pre-gastrulation embryos.

Knowledge detailing human pre-gastrulation embryonic development including spatial self-organization and cell type ontogeny remains limited by available two-dimensional technological platforms  that do not recapitulate the in vivo condition .

Here, they report a three-dimensional (3D) blastocyst-culture system, which enables human blastocyst development through primitive streak anlage (PSA). These 3D embryos mimic in vivo developmental landmarks and 3D architectures, including embryonic disc, amnion, basement membrane, primary and primate unique secondary yolk sac, anterior-posterior polarity formation and PSA.

Using single-cell transcriptome profiling, They delineate ontology and regulatory networks underlying epiblast, primitive endoderm and trophoblast segregation. Compared to epiblast, the amniotic epithelium shows unique and characteristic phenotypes. After implantation, specific pathways and transcription factors trigger differentiation of cytotrophoblasts, extravillous cytotrophoblasts and syncytiotrophoblasts. Epiblasts undergo pluripotency transition upon implantation and maintain its transcriptome until PSA generation driven by different pluripotent factors. Using them 3D-culture approach, They findings elucidate the molecular and morphogenetic developmental landscape during human embryogenesis.

 

Read more,please click:https://www.nature.com/articles/s41586-019-1875-y

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