α-methylacyl-CoA racemase (AMACR), an enzyme localized in peroxisomes and mitochondria, is involved in the β-oxidation of branched-chain fatty acids and fatty acid derivatives. AMACR has been reported to be a biomarker for prostate cancer. The expression of AMACR is also related to other types of cancers such as hepatocellular carcinoma, noninvasive bladder cancer, colorectal cancer and gastric adenocarcinoma.
Abbkine AMACR Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody was tested to react with Human, Mouse and Rat Samples. It’s the best choice for customers who plan to do IHC-P or WB. The Isotype is Mouse IgG1. Abbkine suggested the starting dilutions are as follows: WB: 1:1000, IHC-p: 1:200. The antibody detects endogenous AMCAR/P504S proteins.
I used Abbkine AMACR Monoclonal Antibody to detect prostate cancer cell line LNCaP and human hepatoma cell line HepG2 cell lysate in western blot. Western blot results showed that AMACR protein was positive in prostate cancer cell line LNCaP and human hepatoma cell line HepG2 cell lysate which was consistent with published literatures. The antibody worked great. The signal produced was very strong and clear. The success of this test lay the foundation of my future research and also save my time.