Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release. It has been implicated in modulating mitochondrial calcium homeostasis and proton flux. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Abbkine Bcl-2 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous total Bcl-2 proteins of Human, Mouse and Rat samples. Tested applications of this antibody include WB and IHC-p. Abbkine suggested the starting dilutions are as follows: WB: 1:1000~2000, IHC-p: 1:200.
I used Abbkine Bcl-2 Monoclonal Antibody for WB at 1/1000 dilution. The detected band is approximately 26 kDa which was the same as the predicted molecular weight. I am very satisfied with Abbkine’s product and found it to be worth the cost. I give them a high recommendation and plan to use them again.