Carcinoembryonic antigen (CEA) describes a set of highly related glycoproteins involved in cell adhesion. CEA is normally produced in gastrointestinal tissue during fetal development, but the production stops before birth. Therefore, CEA is usually present only at very low levels in the blood of healthy adults. However, the serum levels are raised in some types of cancer, which means that it can be used as a tumor marker in clinical tests. Serum levels can also be elevated in heavy smokers. Regions of high CEA levels in the body can be detected with the monoclonal antibody.
Abbkine Carcinoembryonic Antigen Monoclonal Antibody is produced to detect endogenous total CEA proteins. The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. It reacts with human samples and the specific applications are IF and IHC-p. Abbkine suggested the starting dilutions are as follows: IF: 1:200, IHC-p: 1:200.
Antibodies to CEA are commonly used in immunofluorescence to identify cells expressing the glycoprotein in tissue samples. In adults, CEA is primarily expressed in cells of tumors. Abbkine Carcinoembryonic Antigen Monoclonal Antibody was used as primary antibody to detect CEA level in human lung cancer tissue with IF analysis. We got the right signal. The result is satisfactory. In the follow-up study, we will, as always, use Abbkine’s products.