Metabolic column Catalase (Catalase) activity detection kit magical efficacy

What is catalase (CAT)? As the name implies, it is an enzyme that catalyzes the decomposition of hydrogen peroxide into oxygen and water. It is present in the body’s peroxide. Catalase is a marker enzyme for peroxisomes, accounting for about 40% of the total peroxisomal enzymes. Catalase is present in all tissues of all known animals, especially at high concentrations in the liver. Catalase is used in the food industry to remove hydrogen peroxide from milk used to make cheese. Catalase is also used in food packaging to prevent food from being oxidized.

Catalase exists in red blood cells and peroxides in some tissues. Its main function is to catalyze the decomposition of H2O2 into H2O and O2, so that H2O2 will not react with O2 under the action of iron chelate to form very harmful- OH. This is one of the reasons why catalase (CAT) has become a key biochemical indicator for researchers.

The role of catalase is to reduce hydrogen peroxide to water: 2H2O2 = O2↑ + 2H2O.

Catalase has multiple functions. People have named them according to their different functions:

  1. Catalase

Catalase (CAT) is an enzyme scavenger, also known as catalase, a binding enzyme that uses iron porphyrin as a prosthetic group. It can promote the decomposition of H2O2 into molecular oxygen and water, remove hydrogen peroxide in the body, and thus protect the cells from H2O2. It is one of the key enzymes in the biological defense system. The mechanism of CAT acting on hydrogen peroxide is essentially the disproportionation of H2O2. Two H2O2 must meet CAT successively and collide with the active center before the reaction can take place. The higher the H2O2 concentration, the faster the decomposition rate.

  1. Decomposing enzymes

This is a stable hydrogen peroxide decomposing enzyme, which can decompose hydrogen peroxide into water and oxygen without affecting fibers and dyes. Therefore, after dyeing after bleaching, H2O2 decomposing enzymes are used to remove the residues on the bleached fabric and the dyeing tank. Hydrogen peroxide to avoid further oxidation of the fibers and oxidation of dyes during dyeing. At the same time, it can shorten the processing time, reduce the water used for washing and reduce the amount of waste water. Especially suitable for yarn, package yarn and knitted fabric. Similarly, with the change of pH value and temperature, the activity of hydrogen peroxide decomposing enzyme changes accordingly, and the activity is greatest around pH7 and 30~40℃. Increasing the concentration of hydrogen peroxide will speed up the decomposition reaction, but it must be noted that when the concentration is greater than a certain amount, the role of the enzyme will be weakened, so that excessive residual H2O2 is detrimental to fibers and dyes. Therefore, the amount of H2O2 cannot be increased arbitrarily because of the H2O2 decomposing enzyme. When using, usually pay attention to the compatibility of H2O2 decomposing enzyme to common surfactants and H2O2 stabilizers. The actual production and application pH is 6~8, temperature is 20~55℃, enzyme dosage is 5 ~ 10 KCLU/liter, time is 10~20 min . This technology has been slowly recognized and accepted in China, and it is very beneficial to improve the vividness of reactive dyes.

In order to carry out qualitative and quantitative research on this indicator, Abbkine has designed a set of kits for professional detection of catalase (CAT) level: KTB1040 CheKine ™ Catalase (CAT) Activity Assay Kit。

Cat NO Product Name Biomarker Function
KTB1040 CheKine™ Catalase (CAT) Activity Assay Kit Catalase (CAT) Quantitative

The kit has the following characteristics:

  • Determination of catalase activity in serum, plasma, tissue/cell lysates and other biological fluids.
  • Catalase activity is directly measured using the catalase’s peroxidation function, and catalase is not interfered by other peroxidases.
  • Extensive detection range: 2-75 µM.
  • Measure catalase activity as low as 2 U/ml.
  • The linearity of the standard product is good, and the coefficient R2 can generally reach more than 0.998.

The research field of this kit is very extensive. In terms of plant research, it mainly includes anti-stress response, photorespiration, mitochondrial electron transport, cold resistance and fatty acid β-oxidation, etc., revealing the microscopic world of plant research and a series of enzyme-catalyzed biochemical reactions. In terms of animal body research, it mainly includes the mechanism of action of gastric digestive enzymes, the intestinal mucosal oxygenated active substance (ROS), the effect of nanoparticles on cell oxidation, and the process of human cell metabolism. In terms of microbial research, it mainly includes strain screening, identification and fermentation to produce catalase, etc., revealing the wonderful world of microbial research and industrial production. In addition, the kit has many applications in food engineering, metabolic engineering, cancer research, etc.

 

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