CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. In humans CD20 is encoded by the MS4A1 gene. CD20 is a 48 kDa intermediate filament protein expressed in mature enterocytes; antibodies to CD20 can be used to study the cellular response to stress. CD20, also called Keratin 21, Cytokeratin 20 or CK20, is another synonym for keratin 20. It has been shown that CD20 plays a role in the microenvironmental interactions of B cells.
Abbkine CD20 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. It detects endogenous CD20 proteins in Human, Mouse and Rat samples. The isotype is Mouse IgG1. The antibody is very suitable for IHC-p. Abbkine suggested the starting dilutions are as follows: IHC-p: 1:200.
Immunohistochemistry can be used to determine the presence of CD20 on cells in histological tissue sections. Because CD20 remains present on the cells of most B-cell neoplasms, and is absent on otherwise similar appearing T-cell neoplasms, it can be very useful in diagnosing conditions such as B-cell lymphomas and leukaemias. My sample is Formalin/PFA-fixed paraffin-embedded human tonsil tissue sections, and I purchased Abbkine CD20 Monoclonal Antibody as primary antibody. The immunohistochemical staining of CD20 was found in the right place, and there was little background. It’s pretty worth enough for me.