CD23 is a 45kDa glycoprotein, which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma.
Abbkine CD23 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous CD23 proteins of Human, Mouse and Rat samples. The antibody was verified suitable for IF and IHC-p. Abbkine suggested the starting dilutions are as follows: IF:1:200, IHC-p: 1:200.
I bought Abbkine CD23 Monoclonal Antibody for Immunofluorescence analysis and the sample were human-stomach tissue sections. CD23 Monoclonal Antibody was diluted just according to Abbkine suggestion at 1:200 (4°C, overnight). IFKine™ Orange Donkey Anti-Mouse IgG was diluted at 1:300 (room temperature, 50min). A positive fluorescence result was virtually 100% specific. I have to say this antibody is worth buying.