CD45 is a type I transmembrane protein that interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type. Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression.
Abbkine CD45 Monoclonal Antibody is produced by immunizing mouse with recombinant protein specific to human CD45 protein. Its applications are WB, IHC-p and IF, with a recommended starting dilution of 1:2000 for WB, 1:200 for IHC-p and IF. The investigator is however advised to determine the optimal working dilutions through experiments. The antibody recognizes endogenous levels of total CD45 protein.
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue using Abbkine CD45 Monoclonal Antibody, it gave a positive signal. I tried the protocol Abbkine suggested. The antibody was diluted at 1:200, incubated with tissue sections at 4°C overnight. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). Goat anti-mouse secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only. Obviously, I got the exact result I hope. It saves a lot of precious time which let my test proceeding under my control.