CDX2, a homeobox domain-containing transcription factor, is a master regulator of the trophoectoderm, the layer that gives rise to extra-embryonic tissues in mammalian development. CDX2 is also involved in intestinal development, and gain of expression or loss of expression has been associated with various human malignancies such as Barret Esophagus and colorectal cancer. Mouse embryonic stem cells deficient in CDX2 display limited hematopoietic progenitor development and altered Hox gene expression, pointing to a role for CDX2 in Hox gene regulation. CDX2 is also implicated in the aberrant expression of Hox genes in human AML cell lines.
Abbkine CDX2 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous levels of total CDX2 protein. Species reactivity of this antibody is human, mouse and rat. Test confirmed the antibody is suitable for WB, IF and IHC-p. The isotype is Mouse IgG1.
Abbkine CDX2 Monoclonal Antibody staining CDX2 in Human colon tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hours at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in PBS containing 5% BSA) for overnight at 4°C. An HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody. The image is shown in the left. The signal was very strong and clear.