Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers . Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. Keratin 7 and keratin 19 are present in hepatic and pancreatic progenitor/stem cells.
Abbkine CK7 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. Supplied in PBS (pH 7.4) containing 0.02% sodium azide as preservative and 50% Glycerol, the antibody detects endogenous levels of total keratin 7 protein of Human, Mouse and Rat samples. Tested applications for this antibody are IF, IHC-p, IP and WB.
Immunofluorescence analysis of human-liver tissue. Abbkine CK7 Monoclonal Antibody was diluted at 1:200 (4°C,overnight). Dylight 549 labled goat anti-mouse was used as secondary antibody. The test result showed a positive fluorescent reaction using the antibody, which provides a basis for our later research. Apart from the antibody’s performance, their customer service was superb. This was especially appreciated.