Keratin, type II cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the KRT8 gene. It is often paired with keratin 18. Keratin 8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. Antibodies to CK8 can be used to differentiate lobular carcinoma of the breast from ductal carcinoma of the breast.In normal tissue, it reacts mainly with secretory epithelia, but not with squamous epithelium, such as that found in the skin, cervix, and esophagus.
Abbkine CK8 Monoclonal Antibody is a high quality mouse monoclonal recommended for detecting endogenous Cytokeratin 8 of mouse, rat and human origin by WB, IF and IHC-P. The isotype is mouse IgG1. Thesuggested starting dilutions by abbkine are: WB: 1:2000-5000, IF: 1:100-200 and IHC-p: 1:200. Optimal working dilutions should be determined experimentally by the investigator.
Abbkine CK8 Monoclonal Antibody staining Cytokeratin 8 in Human colon tissue by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formalin and subjected to heat-mediated antigen retrieval in citrate buffer (0.1M Sodium Citrate) prior to blocking with 5% serum for 1 hour at 4°C. The primary antibody was diluted 1/200 in 5% goat serum in PBS and incubated with the sample for 12 hours at 4°C. Goat anti-mouse was used as the secondary antibody. I have to say I was lucky. This product met my demands. It deserves to recommend.