COL1A1（collagen type I alpha 1 chain) encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for COL1A1.
Abbkine Collagen I Mouse Monoclonal Antibody (4H10) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The immunogen of this antibody is Synthetic Peptide of Collagen I. This antibody could be applied to IF and IHC-p experiments. It can react with human, rat and mouse samples. The original concentration of this antibody is 1mg/ml. During the experiment, optimal working dilutions should be explored by the researchers. The suggested starting dilution for IHC-p is 1:50-200.
Two weeks ago, I ordered the Collagen I Mouse Monoclonal Antibody from Abbkine and want to conduct immunofluorescence analysis in rat kidney tissue. This is my first time to use Abbkine antibody, so I just want to try it. Quite unexpectedly, it brings me a good experience. I received the product soon after the order. During the experiment, they also provide careful and professional guidance. Finally, the fluorescence signal produced is strong and the results are accord with my experiment expectation. The antibody has good specificity, reliable quality and the price is cheaper. I want to recommend it to my friends.