Collagen IV (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a ‘chicken-wire’ meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
Abbkine Collagen IV Mouse Monoclonal Antibody (8E5) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The immunogen of this antibody is synthetic peptide of Collagen IV. This antibody could be applied to IF and IHC-p experiments. It can react with human, rat and mouse samples. The original concentration of this antibody is 1mg/ml. During the experiment, optimal working dilutions should be explored by the researchers. The suggested starting dilution for IHC-p is 1:50-200.
I conducted immunofluorescence analysis in mouse spleen tissue by using Abbkine Collagen IV Mouse Monoclonal Antibody. The antibody was diluted at 1:200. The fluorescence signal produced is strong and the results are accord with my experiment expectation. This is my first time to use Abbkine antibody, and find this is a good brand. The antibody has good specificity, reliable quality and the price is cheaper. I want to recommend it to my friends.