ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.
Human CD54 ELISA Kit employs a two-site sandwich ELISA to quantitate CD54 in samples. An antibody specific for CD54 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD54 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD54 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD54 bound in the initial step. The color development is stopped and the intensity of the color is measured.
We always see many brands who provide Elisa Kits, so it maybe a little difficult to choose an appropriate one. Abbkine maybe a good selection for you. I ever purchased several antibodies form Abbkine, and have to say this is a trustworthy brand. Recently, I ordered a EliKine™ Human CD54 ELISA Kit and want to detect the CD54 in the serum sample. Experiments show that Abbkine ELISA kit has high sensitivity and excellent specificity. No significant cross-reactivity or interference between Human CD54 and analogues was observed. I really appreciate this experience, I will continue to support Abbkine products.