EGF encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. The protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. EGF is proposed to affect growth and/or differentiation of many other fetal and adult tissues.
Human EGF ELISA Kit employs a two-site sandwich ELISA to quantitate EGF in samples. An antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Following the suggestion of my friend, I purchased the EliKine™ Human EGF ELISA Kit from Abbkine. I have to say this is a trustworthy brand. I detected the CD54 in the serum sample. Experiments showed that Abbkine ELISA kit has high sensitivity and excellent specificity. No significant cross-reactivity or interference between Human EGF and analogues was observed. The standard curve is very perfect. I really appreciate this experience, and I will continue to support Abbkine products.