VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.
EliKine™ Rat VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF in samples. An antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
My group purchased EliKine™ Rat VEGF ELISA Kit to detect VEGF in Rat serum. Compared to other Rat VEGF ELISA Kits in the market, Abbkine kit gives a high performance. The Biotin- Streptavidin amplification system let the whole assay more sensitive. It shows no weird result according to the operation manual. It took about 4h to complete the whole assay. This kit is in line with my expectations. I’ll try Abbkine other products in the near future.