DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals. Excision Repair Cross Complementing 1 (ERCC1) forms a complex with ERCC4/XPF, which acts as the 5’endonuclease required to excise the lesion. ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) and involved in repair of double strand breaks. Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC).
Abbkine ERCC1 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects human ERCC1 proteins and was validated for WB. Abbkine suggested the starting dilutions for WB was 1:1000, but the optimal working dilutions should be determined experimentally by the end user. The concentration of the antibody is 1mg/ml.
Western blot analysis of extracts from HeLa and HepG2 cells using Abbkine ERCC1 Monoclonal Antibody at 1/1000 dilution. The observed band size was about 36 kDa. Apart from the performance of the antibody, Due to the excellent communication, this overseas divide did not pose an issue other that the usual annoyances of having to ship materials internationally. The product and service are well worth the money.