The FH gene provides instructions for making an enzyme called fumarase (also known as fumarate hydratase). Fumarase participates in an important series of reactions known as the citric acid cycle or Krebs cycle, which allows cells to use oxygen and generate energy. Specifically, fumarase helps convert a molecule called fumarate to a molecule called malate. Mutations in FH can cause fumarase deficiency and lead to progressive encephalopathy.
Abbkine FH Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody has been validated for WB, IF, IHC-P and tested in Human, Mouse and Rat. Abbkine suggested the starting dilutions are as follows: WB: 1:3000, IF, IHC-P: 1:200. The optimal working dilutions should be determined experimentally by users.
Immunofluorescence image of Abbkine FH Monoclonal Antibody (ABM40073) stained human liver cancer tissue sections. The sections were incubated with ABM40073 at 5µg/ml over night at 4°C. The secondary antibody was (red) IFKine red anti-mouse IgG. DAPI was used to stain the cell nuclei (blue). Target protein signal was strong.