Galectins are a family of β-galactose binding proteins. Galectin-3/LGALS3 is involved in several diverse biological functions. Galectin-3/LGALS3 binds IgE. Galectin-3/LGALS3 production has been shown to increase during inflammation and in obesity, and the protein itself can have an inflammatory effect under certain conditions. Galectin-3/LGALS3 forms a complex with α3, β1 integrin to act as a surface receptor on endothelial cells for the NG2 proteoglycan, which triggers cell motility and angiogenesis. In addition to these functions, galectin-3/LGALS3 is also a required factor for the terminal differentiation of epithelial cells.
Abbkine Galectin-3 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody recognizes endogenous levels of total galectin-3/LGALS3 protein of human samples, and it’s suitable for IHC-p and WB. Abbkine suggested the starting dilutions are as follows: WB: 1:2000, IHC-p: 1:200. But optimal working dilutions should be determined experimentally by the investigator.
I used Abbkine Galectin-3 Monoclonal Antibody staining Galectin 3 of Human ovarian carcinoma tissue by IHC-p. After antigen retrieval and tissue block, tissue sections were incubated with the Galectin-3 monoclonal antibody (1:200) overnight at 4℃. The antibody performance well. Target proteins are found in which they locate. This antibody is deserved to recommended.