The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape. In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes.
Abbkine GFAP Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous levels of total GFAP proteins. It reacts with Human, Mouse and Rat. The tested applications are WB, IF and IHC-p. Supplied as liquid solution, the concentration of the antibody is 1 mg/ml.
GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes. In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system. Abbkine GFAP Monoclonal Antibody as primary antibody was applied in IHC-p of human brain sections. Astrocytes are clearly and strongly labelled. The result is convincing.