The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. In most species, Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms.
Abbkine Histone H3 (Acetyl Lys9) Polyclonal Antibody detects endogenous levels of Histone H3 only when acetylated at lysine 9 in Human, Mouse and Rat samples. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from human Histone H3 around the acetylation site of K9. at AA range: 1-80. It works for WB, IHC-p, IF and ELISA.
My sample is mouse embryonic fibroblasts and I purchased Abbkine Histone H3 (Acetyl Lys9) Polyclonal Antibody for WB. The antibody works well at the recommended concentration 1:2000. The secondary antibody I chose was also from Abbkine(CAT#A21020). This is my first time using Abbkine’s products, and I think Abkkine will be a perfect supplier in my future research life.