The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Histone acetylation occurs mainly on the amino-terminal tail domains of histones H2A (Lys5), H2B (Lys5, 12, 15, and 20), H3 (Lys9, 14, 18, 23, 27, 36 and 56), and H4 (Lys5, 8, 12, and 16) and is important for the regulation of histone deposition, transcriptional activation, DNA replication, recombination, and DNA repair.
Abbkine Histone H4 (Acetyl Lys16) Polyclonal Antibody recognizes endogenous levels of histone H4 protein only when acetylated at Lys16 in Human, Mouse and Rat samples. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from human Histone H4 around the acetylation site of K16. It works for WB, IHC-p, IF and ELISA.
Western Blot analysis of KB cells using Abbkine Histone H4 (Acetyl Lys16) Polyclonal Antibody at 1:1000 dilution. Secondary antibody (catalog#：A21020) was diluted at 1:20000. I observed the band size 11 kDa which is the same as predicted. Whether the price or the performance, this antibody is just what I want.