The family of Heterochromatin Protein 1 (HP1) (“Chromobox Homolog”, CBX) consists of highly conserved proteins, which have important functions in the cell nucleus. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. Members of the HP1 family are characterized by an N-terminal chromodomain and a C-terminal chromoshadow domain, separated by a Hinge region. HP1 is also found at euchromatic sites, where its binding correlates with gene repression. The protein encoded by CBX3（chromobox 3) binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5′ UTR, have been found for CBX3.
Abbkine HP-1γ Mouse Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been verified in IF, IHC-p, WB experiments and shows good effects. You can detect endogenous HP-1γ protein in the samples of Human, Mouse and Rat. About the dilutions, you need to design pre-experiment to determine the optimal concentration, which suits for your specimen.
As researchers, we all know that choosing an appropriate antibody is very important step for the success of an experiment. And I believe Abbkine antibody is a good choice for you. Two months ago, I purchased a HP-1γ Mouse Monoclonal Antibody from Abbkine to finish my graduation thesis. It’s very convenient to order items and I received the product very soon. Compared with other famous companies, the price is quite cost-effective with the same quality. During the process of my experiments, I met some problems. The technical staff offered me very professional guidance and help me analysis the reasons with great patience. Finally, I obtained satisfied results and graduated smoothly. Through the story of my experience, I strongly recommend AbbKine products for you.