NBR1 is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of NBR1 results in multiple transcript variants. The protein encoded by NBR1(autophagy cargo receptor) was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates.
Abbkine NBR1 Mouse Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody has been applied to IF, IHC-p. The antibody can react with Human, Mouse and Rat samples.The investigator should confirm the optimal working dilutions through the pre-test before the formal experiments. Abbkine suggest you choose the following starting dilution: IHC-p: 1:50-200.
I want to share some experiences for you researchers, and hope you could get good results. I detected the NBR1 protein in rat lung tissue by Immunohistochemical analysis. In my experiment, the antibody is diluted at 1:200. I have to say that the Abbkine NBR1 Mouse Monoclonal Antibody is excellent. The images are perfect and I intend to use them to publish my papers. In addition, the price is very competitive. I believe you won’t regret it.