The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . Acetylation appears to play a positive role in the accumulation of p53 protein in stress response . Following DNA damage, human p53 becomes acetylated at Lys382 (Lys379 in mouse) in vivo to enhance p53-DNA binding.
Abbkine p53 (Acetyl Lys382) Polyclonal Antibody are produced by immunizing Rabbits with a synthetic acetylated peptide corresponding to residues surrounding Lys382 of human p53. The antibody detects endogenous levels of human p53 only when acetylated at lysine 382 and suitable for WB, IHC-p, ELISA.
Abbkine p53 (Acetyl Lys382) Polyclonal Antibody is used to stain human breast adenocarcinoma formalin fixed paraffin embedded tissue sections, 10μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated secondary antibody. The target staining is very clear and the sensitivity of the antibody was perfect.