p53, also known as Cellular tumor antigen p53 or Tumor suppressor p53, was discovered as a cellular protein associating with the transforming protein of SV40 tumor virus. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells. Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy. Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines.
Abbkine p53 Monoclonal Antibody (Mouse Monoclonal Antibody) was validated in IF, IHC-p and WB to detect endogenous p53 proteins of human samples. Abbkine suggested the starting dilutions are as follows: WB: 1:2000, IHC-p: 1:200, IF: 1:100-200. The antibody gave a positive signal in most human tissue, such as Human colon adenocarcinoma tissue sections as well as some whole cell lysates.
Mutations of the p53 protein have some characteristic features. They exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation. We used Abbkine p53 Monoclonal Antibody staining p53 in human colon adenocarcinoma formalin-fixed paraffin-embedded tissue sections. DAB was used as the chromogen. Positive signal was gotten using the antibody. In view of the good performance of this antibody, I will try to use Abbkine other products.