PDGFRα, platelet-derived growth factor receptor alpha, also termed PDGFRA, encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. This receptor binds to certain isoforms of platelet-derived growth factors (PDGFs) and thereby becomes active in stimulating cell signaling pathways that elicit responses such as cellular growth and differentiation. The receptor is critical for the development of certain tissues and organs during embryogenesis and for the maintenance of these tissues and organs, particularly hematologic tissues, throughout life. Mutations in PDGFRA have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
Abbkine PDGFRα Mouse Monoclonal Antibody (7A3) takes synthetic peptide of PDGFRα at AA range of 1010-1090 as immunogen. The antibody was affinity-purified from mouse ascites by affinity-chromatography. The antibody could be used to detect endogenous levels of PDGFRA in the samples of human, rat and mouse. It has been validated in IF and IHC-p experiments. Suggested starting dilutions are: IHC-p: 1:100-200, and the researchers should explore optimal concentrations for your own tests.
Our lab bought this antibody one week ago. I stained mouse brain sections at a dilution 1:200 and there are strong signals in corpus callosum. In one word, this antibody is an excellent antibody. Also, my other colleague conducted the immunohistochemical analysis of rat spleen tissue. PDGFRα Mouse Monoclonal Antibody (7A3) was diluted at 1:200. It works well for IHC staining.