allosteric effectors and covalent modification. Protein. Kinase A. Alanine would indicate the plenty of protein building blocks are present for the production of macromolecules, e.g., proteins. The hormone glucagon stimulates a protein kinase A, which. phosphorylates pyruvate kinase, converting it to a less active form.

Pyruvate Kinase and Glycolysis

 

PK (EC 2.7.1.40) is widely present in animals, plants, microorganisms and cultured cells, and catalyzes the last step reaction in the glycolysis process. It is one of the main rate-limiting enzymes in the glycolysis process and the key to ATP production. It is important to measure PK activity. Pyruvate kinase (PK) is ubiquitously expressed across all living beings.

 

Pyruvate Kinase is an enzyme that is involved in glycolysis. Glycolysis is highly regulated at three of its catalytic steps: the phosphorylation of glucose by hexokinase, the phosphorylation of fructose-6-phosphate by phosphofructokinase, and the transfer of phosphate from PEP to ADP by pyruvate kinase.

allosteric effectors and covalent modification. Protein. Kinase A. Alanine would indicate the plenty of protein building blocks are present for the production of macromolecules, e.g., proteins. The hormone glucagon stimulates a protein kinase A, which. phosphorylates pyruvate kinase, converting it to a less active form.

(Picture Source: https://slideplayer.com/slide/7426697/ )

 

Through year of efforts, Abbkine research scientists have developed a series of assay kits for cell metabolism and that constitutes Abbkine CheKine™group. For detailed index, you are welcome to view at https://www.abbkine.com/?s=CheKine%E2%84%A2&post_type=product&submit=. In which we can see the detection kits of NAD/NADH, NADP/NADPH, superoxide dismutases (SOD), peroxidase (HRP) and glucose, but today we are going to introduce CheKine™ Pyruvate Kinase (PK) Colorimetric Assay Kit for you.

 

To more specific, the benefits of Abbkine Pyruvate Kinase Colorimetric Assay Kit can be concluded as below:

  1. Various sample compatibility: Determination of Pyruvate kinase (PK) activity in cells, bacteria, serum (plasma), tissue/cell lysates and other biological fluids.
  2. Kit components and procedures are simple.
  3. Sample preparation, experimental steps and result calculation are more detailed and accurate.
  4. Long shelf life.

 

You can view the kit protocol at https://www.abbkine.com/file/booklet/KTB1120-B.pdf .

 

Abbkine is aimed at to provide high quality and cost effective reagents to global scientists, this  Pyruvate Kinase Colorimetric Assay Kit has been optimized to use and can deduct your experiment cost. For detailed product information, you can refer in below sheet.

CAT# Product Name Size and Price
KTB1120 CheKine™ Pyruvate Kinase (PK) Colorimetric Assay Kit

 

$100/48T

$180/96T

 

If you are interested in Abbkine CheKine™ Pyruvate Kinase (PK) Colorimetric Assay Kit, feel free to contact service@abbkine.com, our service representative will get back to you shortly!

 

About Abbkine Scientific Co., Ltd.

Abbkine offers services for global scientists in the fields of proteomics and cytology, and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, to promote the development of fields including life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, to help your research career!

 

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