Abbkine Scientific is one of the most trusted names in the field of life science research, providing innovative research tools and products since it was founded some years back. The Chinese based scientific research company recently announced the official launch of its new product – the CD5 Monoclonal antibody.
The CD5 antibody as it is also referred to as is another product from the stables of the research giant. The product like many other tools from Abbkine Scientific is designed to help simplify and enhance research processes, leading to more effective results.
The T-cell surface glycoprotein CD5 antibody has come under scrutiny from different quarters, especially researchers and investigators alike. Below is a review of the product, detailing the features, benefits, pros and cons of the newly-launched antibody.
Overview of CD Monoclonal Antibody
CD5 also designated as Lyt-1 is a popular substance famous for being a trans-membrane glycoprotein, which is expressed on 70% of normal peripheral blood lymphocytes. It has also been identified on practically all T lymphocytes in thymus and peripheral blood.
In addition, the activation of T cells via the T cell receptor also known as TCR leads to tyrosine phosphorylation of CD5 and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation.
CD5 has been discovered to associate with TCR/CD3-z chain, and the Src family kinase Lck p56. Vitro studies have revealed a significant increase in the kinase activity of Lck bound to CD5, by as much as ten to fifteen folds.
CD72, which is the B cell antigen, serves as a receptor for CD5. The effect of CDG binding with its cognate receptor is not readily known at the moment. However, report has it that it plays a role in the selection of thymic.
Features of the CD Monoclonal Antibody
The CD5 Monoclonal antibody comes with a number of features or properties. While it shares some of the features with its counterparts, it possesses some unique attributes that distinguishes it from other such products.
Some of the features are mentioned below
- Formulation – liquid solution
- Concentration – 1 mg/ml
- Immunogen – synthetic peptide
- Host – Mouse
- Reactivity – Human, Mouse, Rat
- Applications – IF, IHC-p
- Isotype – Mouse IgG1
- Clonality – Monoclonal
- Storage buffer – PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
One of the unique features of the CD5 monoclonal antibody is the method of purification employed while producing it, as it is affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Another relatively unique feature of the antibody is the ability of investigators to determine the best working dilutions that suits the purpose of the experiment. While the manufacturer suggests starting dilutions of IHC-p: 1:200, IF: 1:200, investigators are advised to determine the optimal working dilutions experimentally.
The Pros of CD5 Monoclonal Antibody
Also known as LEU1 antibody, the product has been identified to have a number of pros especially when compared with other antibodies in its category. One of the pros of the product is its durability as users can store it for as long as one year at -20°C from date of shipment.
Another advantage of the product is its ability to detect endogenous CD5 proteins.
Its availability in a liquid solution and flexible dilutions also allows for flexibility for investigators and researchers alike.
The Cons of CD5 Monoclonal Antibody
Currently, there are no known negative effects or cons of the CD5 Monoclonal Antibody. However, the manufacturers have warned against using it for human or clinical diagnosis, as it is strictly for research purposes only.
The CD5 Monoclonal Antibody is another great product from Abbkine Scientific Company Limited and tends to satisfy the desire of most scientific investigators and researchers.