VIM Polyclonal Antibody Review

Vimentin is a protein that in humans is encoded by the VIM gene. The gene encoding human vimentin is located on the short arm of chromosome 10. Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.

VIM Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB, IHC-p, IF and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:100-1:300, IF: 1:200-1:1000, ELISA: 1:10000.

I applied VIM Polyclonal Antibody in immunohistochemical analysis of paraffin-embedded human-stomach-cancer tissue. Vimentin, the sign of specificity of normal mesenchymal cells and their tumors, can co-express with cytokeratin in many epithelial cells and their tumors. The quality of the VIM Polyclonal Antibody is found satisfactory. I am very happy that I chose this product.

 

VIM Polyclonal Antibody
  • Editor Rating

  • Rated 5 stars
  • Spectacular
$100

  • Product Features
    Editor: 100%
  • Usage Performance
    Editor: 100%
  • Price Advantage
    Editor: 100%

Review Summary:

I applied VIM Polyclonal Antibody in immunohistochemical analysis of paraffin-embedded human-stomach-cancer tissue. Vimentin, the sign of specificity of normal mesenchymal cells and their tumors, can co-express with cytokeratin in many epithelial cells and their tumors. The quality of the VIM Polyclonal Antibody is found satisfactory. I am very happy that I chose this product.

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